Cajal course: Advanced Imaging Methods for Cellular Neuroscience
September 10th, 2018
to September 28th, 2018
About the Event
Brain cells, and especially neurons, have developed a high degree of polarisation as well as micro- and nanoscale compartmentalisation of cellular components when compared to other cell types. Cellular neuroscience has benefited in the last years from the development of sophisticated high-resolution imaging techniques including subcellular nanoscopy and the development of novel probes that allow the visualization of neuronal architecture and function in vitro and in the brain in vivo. These advances have placed cellular neuroscientists at the forefront of cell biology and have allowed them to study at unprecedented spatial and temporal resolution processes such as synaptic transmission, receptor trafficking, or morphological adaptations during brain plasticity in diverse models ranging from cultured neurons to the entire brain. The course and its participants will greatly benefit from the infrastructure provided by the Bordeaux Imaging Centre. Finally, this Course will emphasize how new techniques can address specific biological issues and lead to new concepts and discoveries in cellular neuroscience.
Modern neuronal cell biology relies on a large array of advanced techniques. The past decade has produced a revolution in technological innovations and the interweaving of approaches at the molecular, cellular, network and organismal levels (“from genes to behaviour”). This advanced course allows the students to obtain hands-on experience with innovative techniques expected to be central in cellular neuroscience in the coming decade. These techniques focus on the study of cell proliferation and migration, axonal growth, cellular trafficking, synaptogenesis as well as mature cell function, in particular synaptic transmission and plasticity. Techniques will include in vitro and in vivo gene transfer (including viral vector technology and single cell electroporation), cell and tissue imaging by confocal imaging and light sheet microscopy, photomanipulation in living tissue and optophysiology, patch clamp electrophysiology, imaging of proteins and lipids by super-resolution microscopy (STED and PALM/STORM), single-particle tracking methodologies, correlative light electron microscopy (CLEM), live imaging of protein interactions (FRET, FLIM). Rodent and human model systems (iPSCs, organoids) are central, and successful invertebrate models such as Drosophila and C. elegans will be also available.