Connecting European Neuroscience

EMBL Course: Advanced training with Oxford Nanopore Technologies

Dates
January 14th, 2019 to January 18th, 2019
Venue
Heidelberg, Germany

Bioinformatics, Biological, Data

About the Event

The goal of this course is to teach nanopore sequencing using the Oxford Nanopore Technologies (ONT) platform. We will start with an introduction into ONT technology and devices, with the goal of covering end to end workflows for the preparation and analysis of human and yeast samples using whole genome and barcoded cDNA sequencing approaches. This course will cover the wet lab preparation of libraries from genomic DNA and total RNA, with a focus on the critical steps and potential pitfalls and understanding what constitutes a ‘good’ sample for purpose of best results using the technology. The training includes an overview of the MinKNOW GUI for GridION and MinION devices. We then cover methods available for basecalling and analysis of samples for structural variants and differential gene expression, using both Oxford Nanopore and open source tools.

Contact

EMBL Events
Meyerhofstraße 1

Phone:+49 6221 387 8797
Email:events@embl.de
Website:https://www.embl.de/training/events/2019/NAN19-01/

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