Job ID: 60158

Research fellow in Neuroscience

Position: Post-doctoral Position

Deadline: 28 September 2021

Employment Start Date: 1 February 2022

Contract Length: 4 years

City: Coventry

Country: United Kingdom

Institution: University of Warwick

Department: School of Life Sciences

Description:

We are seeking a highly determined and motivated candidate to join a team working on an innovative project to investigate a novel hypercapnic arousal pathway in the mammalian brain. Arousal in response to raised CO2 is a key survival pathway. We have found that CO2 sensitive connexin 26 (Cx26) hemichannels are expressed by GABAergic neurons in the VTA.  We propose that the opening of these hemichannels by raised CO2 levels will shunt the GABAergic neurons removing inhibition and leading to arousal. This BBSRC-funded project will use a number of approaches to investigate this pathway, including a dominant negative construct to remove CO2-sensitivity from the Cx26 hemichannels expressed in the VTA neurons.

 

The Research Fellow will initially use the dominant negative construct to confirm that the CO2 sensitivity of VTA neurons is from Cx26 hemichannel expression. The construct will then be used in in vivo experiments to determine effects on arousal in response to raised levels of CO2.  By using a variety of tracing methods combined with whole cell path clamp recording, the components of this novel arousal circuit will be fully defined.

 

The successful Research Fellow candidate will have a PhD in a relevant area of experimental sciences, a career-appropriate track record of publishing high quality papers, and a strong intellectual interest in understanding neurophysiological signalling mechanisms by tracing signalling pathways from fundamental mechanism through to the final physiological output. The Research Fellow post requires a diverse skill-set. The successful candidate should be keen to learn new techniques but should have expertise in whole cell patch clamp recording methods, imaging with fluorescence/confocal microscopy. Some molecular biology knowledge, possession of a personal licence and experience of in vivo procedures operating under a UK Home Office licence will be an advantage.