Job ID: 63365

Postdoc at Nencki Institute of Experimental Biology PAS

Position: Post-doctoral Position

Deadline: 19 December 2021

Contract Length: 3 years

City: Warsaw

Country: Poland

Institution: The Nencki Institute of Experimental Biology PAS

Department:

Description:

The Nencki Institute of Experimental Biology PAS is seeking a candidate for the position of: Postdoc

Number of available positions: 1

Place of work: Laboratory of Cell Biophysics

Researcher career profiles: R2
Field of science: exact and natural sciences

Type of recruitment: National Science Centre Sonata BIS project

Requirements:

Education: PhD in chemistry, biology, biotechnology, neurobiology or similar received < 7 years ago

professional experience: experience in experimental work in the laboratory, strong publication record

skills: creativity and analytical thinking, experience in the application of methods of cell biology, biochemistry, proteomics, mass spectrometry analysis or related. Skills in genetic manipulations or experience in animal handling and behavioral studies are additional attributes.

competences: ability to work both independently and as a part of a team, independence in planning, performing experiments and data analysis, excellent verbal communication skills

Scope of tasks:

Description of the role and the time-course of S-PALM in learning and memory and high-resolution analysis of brain palmitoylome

Project description:

The hallmark of the mammalian brain is the ability of processing and storing information in synapses and highly organized neuronal networks. The brain can adapt and change in response to various events under physiological conditions (i.e. learning) and pathophysiological conditions (i.e. epilepsy, stroke). Understanding the fundamental mechanisms of the plasticity of synaptic connections and neuronal network code lies in the center of contemporary neurobiology, neuropharmacology, and medicine. Targeting proteins to membranes is aided by covalent and reversible addition of fatty acids such as palmitic acid (S-palmitoylation). This modification may affect the membrane localization of the protein, protein activity and protein-protein interactions. We hypothesize this process plays a key role in learning and memory, because pharmacological modification of S-palmitoylation in rat nervous tissue abolished long-term synaptic potentiation, a substrate of memory formation. We have also discovered, that spatial learning in rats is associated with S-palmitoylation of specific neuronal proteins.

The project aims at describing the interaction of neurotransmitter dopamine and S-palmitoylation of synaptic proteins in the hippocampus- part of the brain playing crucial role in learning. In the team consisting of experts of various fields, we combine multiple experimental techniques: detection of modified proteins with mass spectrometry, genetic and pharmacological manipulation of activity of enzymes related to palmitoylation, behavioral  analysis of learning in animals. We perform live recordings of electric signals from synapses, single neurons and neuronal networks and currents conducted by synaptic proteins to better understand the functional aspects of palmitoylated vs non-palmitoylated states.

Taking advantage of various experimental models and advanced quantitative methods we plan to address the following questions:

  • What is the role and time-course of S-palmitoylation in synaptic plasticity?
  • How dopamine affects S-palmitoylation of synaptic proteins?
  • What is the effect of S-palmitoylation of the function of proteins involved in learning and memory?

The results of this project will shed new light on the general mechanisms governing brain plasticity and may be also important for the understanding of dopamine role in brain physiology as well as planning new therapeutic strategies for the treatment of some neurological diseases.

Employment conditions:

We offer:

  • Type of contract: permanent position
  • Duration of contract: 3 years
  • Commencement of employment:
    • Work in an young team in an excellent scientific environment
  • Access to the state-of-the-art equipment and facilities dedicated to neuroscience research
    • Participation in courses and conferences
    • Comprehensive training in electrophysiological recordings
    • Training and visits in collaborating laboratories in Poland and abroad
  • Additional benefits: medical insurance, possibility to use sport card, social fund. The Institute is located at Ochota academic campus close to the center of the city with free bicycle and car parking lot.

 Required documents: CV, phd diploma, description of professional experience with emphasis on research techniques used by the applicant, list of publications, letter-of-intent, references

You can use the Polish equivalent of a foreign academic degree if it meets at least one of the following criteria:

  • is covered by the provisions of an international agreement on the equivalence of education,
  • was issued by an authorized institution operating in an EU, OECD or EFTA Member State,
  • was issued in another country and recognized on the basis of the regulations on the recognition of academic degrees obtained abroad (learn more about the procedure for the recognition of academic degrees: https://nawa.gov.pl/en/recognition/recognition-for-professional-purposes/recognition-of-scientific-degrees-and-titles

The documents should be sent  to postdoc@nencki.edu.pl with note “postdoc TW”, the deadline is Dec 19th, 2021

Link to website: https://www.nencki.edu.pl/laboratories/laboratory-of-cell-biophysics/

Additional information:

Please include the following clause in your CV:

“I give consent for my personal data included in the recruitment form to be processed by Nencki Institute of Experimental Biology PAS with its registered seat in Warsaw at Pasteur Street No. 3 (data controller) for the purposes of recruitment for postdoc position pursuant to Regulation (EU) 2016/679 of the European Parliament and of the Council of 27 April 2016 on the protection of natural persons with regard to the processing of personal data and on the free movement of such data, and repealing Directive 95/46/EC in compliance with a legal obligation to which the data controller is subject to (Art. 6 section 1 letter a) and the Act on personal data protection of 10 May 2018 (Journal of Laws of 2018, item 1000).”

 

Information Clause

The Controller of data is Nencki Institute of Experimental Biology PAS with its registered seat in Warsaw at Pasteur Street No. 3.

Contact Details of the Personal Data Protection Officer: iod@nencki.edu.pl

Personal data in the recruitment form is collected and will be processed only for the purposes of recruitment for post-doc position conducted by Nencki Institute of Experimental Biology PAS.

Giving consent for processing of your personal data is voluntary; however, it is necessary in order to take part in recruitment. I hereby state that I was informed about the right to withdraw my consent at any time. The withdrawal of the consent does not influence the lawfulness of any processing performed before the consent withdrawal.

Personal data collected in order to perform the recruitment process shall be processed throughout the period of time necessary for the organisation and completion of the recruitment process, as well as for considering a possible complaint; however, not longer than up to 31.03.2022

Personal data will not be transferred to a third party.

You have the right to request the data controller for access to your personal data, to edit or limit its processing, as well as to raise an objection or transfer your data. You are also entitled to file a complaint to the supervisory body (President of the Office for Personal Data Protection)