Job ID: 64321

Two postdoctoral positions on modeling autism spectrum disorders using iPSC derived neuronal models

Position: Post-doctoral Position

Deadline: 15 January 2022

Employment Start Date: 1 April 2022

Contract Length: 3 years

City: Nijmegen

Country: Netherlands

Institution: Radboud University Medical center

Department: Human genetics

Description:

Two postdoctoral positions are available for a fixed term of 3 years to perform research on modelling autism spectrum disorders using iPSC derived neuronal models in 2D. The projects will capitalize on a hiPSC phenotyping platform that we have established and which is based on the differentiation of patient-derived neurons on micro-electrode arrays (MEAs), to interrogate the functional consequences of ASD-linked variants at the level of E/I balance (Frega et al., 2019; Gunnewiek et al., 2020; Linda et al., 2020; Mossink et al., 2021a). You will  combine network measurements with transcriptomic data (single nuclei transcriptomics) in the same experiment to identify molecular mechanisms underlying specific network phenotypes. You will model a large representative panel of 18 rare ASD variants, validate variants of unknown significance (VUS) and develop/test genetic rescue strategies.

The research will be located at the Human genetics Department (Nadif Kasri group) and embedded in the Donders Institute for Brain, Cognition and Behaviour of the Radboud University Medical Center.

Our research team at the Radboud university medical center is renowned for its genetic, molecular and electrophysiological research in the field of neurological and neurodevelopmental disorders. Over the past years we have developed unique knowledge and resources, such as the human (patient) induced pluripotent stem cell lines, including the required experimental platforms for in-detail assessment of the derived human neuronal in-vitro network models.

 

  1. Tasks & Responsibilities 
  • You will be responsible for the functional characterisation of IPSC-derived neuronal networks from ASD patients.
  • You will be responsible for developing novel therapeutical strategies for selected ASD cases
  • You will be responsible for integrating electrophysiology data with transcriptomics data (MEA-seq)
  • Designs and performs scientific research
  • Works in a team with PhD students, scientists and clinicians
  • Discusses, plans and performs research in a stimulating environment
  • Works in a highly international research group and is willing to travel to other institutes within Europe.

 

  1. Profile

Postdoc position 1:

  • Phd in molecular life sciences, biomedical sciences or a related discipline.
  • A strong background in molecular and cellular neuroscience
  • Experience in experience in single cell (patch clamp) and /or network electrophysiology (MEAs)
  • Experience with cell culture of primary neurons and induced pluripotent stem cells

 

Postdoc position 2:

  • Phd in molecular life sciences, biomedical sciences or a related discipline.
  • A strong background in bioinformatics
  • Experience with analysing transcriptomics data and data integration