Job ID: 78540

Postdoctoral Researcher at MMRI in Warsaw, Alzheimer’s disease, iPSC reprogramming, cerebral organoids

Position: Post-doctoral Position

Deadline: 22 April 2022

Employment Start Date: 22 April 2022

Contract Length: 36 months

City: Warsaw

Country: Poland

Institution: Mossakowski Medical Research Institute Polish Academy of Sciences

Department: Department of Neurogenetics and Functional Genomics

Description:

         MMRI is seeking a postdoctoral candidate interested in studying pre-symptomatic synaptic dysfunction in Alzheimer’s disease. We are starting a new international project named PRESSAD in frames of JPco-fuND2 Call 2021 program. Project partners are from France,  Sweden, Netherlands and Hungary.

In the PreSSAD project, we aim at addressing presymptomatic synaptic deficits in the context of the human Alzheimer’s disease pathology, by combining the expertise of five European groups spanning from the identification of CSF biomarkers in preclinical AD cohorts to human synaptic biology. A major originality of the proposal is in the use of innovative human biological samples: 1) using iPSCs-derived human neurons with altered expression of presynaptic proteins (e.g. called SNARE proteins) in mouse neocortex, 2) human iPSCs-derived cerebral organoids and 3) organotypic cortical cultures obtained from human surgical resections. All models of human neurons and circuits will be genetically targeted to assess the early physiopathological stages of synapse dysfunction and loss, in combination with a proteomic analysis of synaptic biomarkers.

We expect to target early physiopathological mechanisms in the progression of AD from these subthreshold changes in the brain that are unnoticeable to the person affected to neurodegeneration and severe cognitive deficits. Through the combined study of biological samples of presymptomatic AD patients to the use of innovative experimental models of human neurons, the PreSSAD project will provide a much improved definition of the timing and magnitude of pathophysiological changes associated with AD with a focus on presynaptic biological markers and mechanisms. We anticipate that our combined data will be critical for the development of diagnostic and predictive tests and for the design of prevention trials. In addition, the innovative methodological development directly targeting human neurons and circuits has the great potential to deliver new cell models for future therapy design, to treat AD, as well as potentially other neurodegenerative diseases.

Description of duties:

  • planning and implementation of research tasks in accordance with the schedule of the project,

  • in detail: study of presynaptic deficiency in cultures of neurons derived from iPSC and in cerebral organoids derived from patients with Alzheimer’s disease carrying PSEN1 or APP mutations (optionally corrected with CRISPR / CAS9), or sporadic patients,

  • designing and conducting experiments with the use of biochemistry, molecular biology, genetic engineering and advanced cell culture, and microscopic imaging methods,

  • analysis, interpretation and integration of project data,

  • presenting the results at the group meetings, international Consortium meetings, external seminars and scientific conferences,

  • preparation of scientific publications,

  • introducing new research technologies and cooperation with a foreign partner involved in the current research project.

Necessary Requirements:

  • PhD degree in biological sciences, neurosciences, pharmacological sciences, medical sciences or relevant, obtained within a period not exceeding 7 years before January 1, 2022*

  • documented scientific achievements including publications in journals from JCR list,

  • participation in scientific conferences and internships,

  • experience in laboratory techniques in biochemistry, molecular biology e.g. DNA, RNA, isolation; Western blot; qPCR; gene overexpression, gene silencing (optionally CRISPR/Cas9); immunohistochemistry; spectrophotometric/fluorometric techniques,

  • experience in planning and conducting in vitro cell cultures (eg. primary cell lines, neural cell lines, reprogramming and culturing of induced pluripotent stem cells (iPS)),

  • ability to work in a team and independently,

  • excellent knowledge of English, allowing effective communication and preparation of scientific manuscripts.

* this period may be extended by the duration of long-term sickness breaks (maximum 90 days) and / or by 18 months for women in connection with maternity / parental leave for 1 child and the number of months of adequate leave for men.

Optional Requirements (Assets):

  • experience in work using a model based on (human) iPSCs-cerebral organoid or iPSCs-neuonal progenitors-neurons for neurodegenerative disorders or other,

  • experience in electrophysiological recording on neurons,

  • experience in substantive care for students and junior scientists.

We offer:

  • fixed term, full-time employment contract,

  • contract for 36 month with possibility of extension,

  • gross salary: around 7800 PLN gross / month,

  • the opportunity to work in a pleased atmosphere, in a dynamic, developmental research group,

  • scientific collaboration with a research partners from France, Sweden, Hungary, and Netherlands and with other Polish and foreign research institutions,

  • opportunity to participate and present obtained results at international conferences.

How to Apply:

Please send your documents to mwezyk@imdik.pan.pl indicating the reference number ZCHZ-111-1/22 in your correspondence.

Required documents:

  • motivation letter with description of candidate’s scientific interests, scientific work, scientific independency, participation in research grants,

  • CV listing candidate’s education, professional experience, scientific achievements, authored or co-authored publications and conference abstracts, internships and training,

  • copy of the PhD (or equivalent) diploma,

  • reference letters.

For more information about the project, please contact dr Michalina Wezyk (mwezyk@imdik.pan.pl)

Expected date of the interview: 26-28.04.2022

Expected decision date: 4.05.2022

Expected job starting date: 9.05.2022